Family name: Angeletti
First names: Silvia
Date of birth: 11 December 1970
Nationality: Italian
Civil status: Married
Education: Degree in Biological Science, 110/110 Lode ; University “La Sapienza” of Rome, on July 1994. Degree in Medicine and Surgery, 110/100 Lode; University Campus Bio-Medico of Rome, on October. 2008 Board in Microbiology and Virology, 70/70 lode; University “La Sapienza” of Rome, on November 1998. Language skills:Good reading, speaking and writing English and Spanish Present position: Researcher in Clinical Pathology at theUniversity Hospital Campus Bio-Medico of Rome, Italy Key qualifications:Director of the Laboratory Division of the University Hospital Campus Bio-Medico, Via Alvaro del Portillo 200, Rome, Italy.

* Infectious disease diagnosis
* Epidemiology of infectious diseases
* Phylogenetic analysis applied to viral and bacterial infections
* Multidrug resistant (MDR) pathogens
* Laboratory markers of inflammation and infection
* Diagnostic and prognostic markers associated to infectious and chronic diseases
* Laboratory quality indicators

Dr. Ram Mohan Ram Kumar is a scientist working at University of Zurich in the field of Cancer Biology. His area of interest is in sarcoma biology. He did his PhD at Universitat Pompeu Fabra, Spain and worked in pancreatic cancer.

Sarcoma Biology

My name is Yadira Prieto Curbelo, I was born in Havana (Cuba) on November 3, 1982. I graduated of bachelor of Biochemistry in 2005 (University of Havana, Cuba). I graduated of bachelor of Biochemistry in 2005 (University of Havana, Cuba). Since 2005 I began to work at Center of Molecular Immunology specifically at Development Process Direction. From 2005 to date, I acquired expertise in different areas, such as: purification of recombinant proteins, monoclonal antibodies; development of analytical techniques for process control of recombinant proteins purified. Also, in proteomic studies of expression stability in recombinant NS0 myeloma cell lines; establishing of quality specifications of the therapeutic vaccines. On the other hand, I developed and validated of chromatographic techniques for the Mab愀 characterization and quantification. I assimilated and standardized of FACS assays. As part of my training I have provided services in mass spectrometric for characterization of recombinant proteins and oligosaccharide residues. The cell culture of the recombinant NS0 cell lines and measurement expression levels of mRNA in recombinant NS0 myeloma cell lines are part of acquired knowledge.

Purification of recombinant proteins, monoclonal antibodies; development of analytical techniques for process control of recombinant proteins purified. Also, in proteomic studies of expression stability in recombinant NS0 myeloma cell lines; establishing of quality specifications of the therapeutic vaccines. On the other hand, I developed and validated of chromatographic techniques for the Mab愀 characterization and quantification. I assimilated and standardized of FACS assays. As part of my training I have provided services in mass spectrometric for characterization of recombinant proteins and oligosaccharide residues. The cell culture of the recombinant NS0 cell lines and measurement expression levels of mRNA in recombinant NS0 myeloma cell lines are part of acquired knowledge.

Joana S. Amaral graduated in pharmaceutical sciences and obtained her PhD in nutrition and food chemistry at the University of Porto in 2006. Currently, she is a professor at the Polytechnic Institute of Braganc¸a teaching biochemistry and molecular and cellular biology, among other issues, and is a member of the Associate Laboratory REQUIMTE. Her current work is related to food quality and food authenticity, including the development of DNA-based methodologies for the detection of adulterations in commercial game meat products.

Application of analytical techniques, mainly chromatography, in the study of nutraceutical and phytochemical compounds in foods. Molecular biology techniques (PCR and conventional Real-Time PCR) applied for food safety and authenticity studies, namely detection of genetically modified organisms (GMOs) and detection of ingredients in processed foods (meat species, botanical authentication of honey, etc.).

Educations:
2007-2011PhD in Molecular Biology and Biotechnology:Department of Biotechnology, Faculty of Biology, University of Leon, Spain
2004-2006 M.Sc. in Molecular Genetics: Department of Biology, Faculty of Sciences, Isfahan University, Isfahan, Iran
2000-2004 B.Sc. in Cellular and Molecular Biology: Department of Biology, Faculty of Sciences, ShahidChamran University, Ahvaz, Iran

Academic Appointment
2015-presentRazi Vaccine and Serum Research Institute, Karaj, Iran; Faculty Member
2014-2015: Tehran University, Tehran, Iran; Lecturer
2012-2013: St. John’s University, NY, USA;Post-Doctoral Research Assistant
2011- 2012: Lehman College (CUNY), NY, USA; Post-Doctoral Research Assistant

Research History
(2015-present): As part of a research line at biotechnology lab ofRazi Vaccine and Serum Research Institute we are studying the epitope mapping of snake venom using monoclonal antibodies.
(2012-2013):In my postdoctoral researches at St. John’s University I studied the interaction network in membranechannel-forming proteins TRPP/PKD complexes. These proteins were previously identified as the disease genes causing autosomal dominant polycystic kidney disease (ADPKD). I have studied the roles of extracellular loops in these proteins for the assembly and function of TRPP/PKD complexes using co-IP, IF and electrophysiological assays. I wasalso involved in other project which studied the effect of Edaravonein neuronal cellprotection in response to oxidative stress. Weperformed comparative two-dimensional gel electrophoresis (2DE) based proteomic analyses on SH-SY5Y neuroblastoma cells exposed to oxidative stress and in combinationwith Edaravone. We showed that Edaravone can reverse the cytotoxic effects of H2O2 through itsspecific mechanism. We observed that oxidative stress changes metabolic pathways and cytoskeletalintegrity. Edaravone seems to reverse the H2O2-mediated effects at both the cellular and protein level viainduction of Peroxiredoxin-2.
(2011-2012): In my former postdoctoral research at Lehman College we performed a comprehensive study on the regulation of ExoR protein expression and its interaction with ExoS protein, the membrane-bound sensor of the ExoS/ChvI two-component regulatory system by using different site directed mutagenesis. In SinorhizobiummelilotiExoR–ExoS/ChvIsignallingpathway regulate the biosynthesis of succinoglycan, an exopolysaccharide important for host invasion. We are in the process of filing a U.S. patent about ExoR/ExoS/ChvI sensing pathway involved in the symbiosis.
(2007-2011):In my PhD studies at INBIOTEC (a Spanish biotech company collaborating with University of Leon), I worked on different projects. My main study was characterization of gamma-butyrolacone receptors in tacrolimus producer strains S. tsukubaensisand S. tacrolimicus and analysis of their autoregulatory function. Using REDIRECT technology I succeed to generate several deletions in the regulatory genes involved in gamma-butyrolactone pathway. Then I analyzed the impact of alteration of these targets on tacrolimus production (using HPLC analysis) and morphogenesis (using electronic microscopy). The expression and purification of gamma-butyrolactone receptor proteins using GST gene fusion system and FPLC and analysis of their DNA binding activity using EMSA and Footprinting assays were other part of my study.
I also optimized the fermentation conditions for tacrolimus production and growth rate in S. tsukubaensisin terms of carbon, nitrogen and calcium sources as well as pH. As a result the tacrolimus production in the optimized condition increase up to four fold. In addition I was involved in the genome sequencing project of S. tsukubaensis where I analyzed the physical map of S. tsukubaensis genome using PFGE electrophoresis. Moreover we compared the secretomic maps of three Penicilliumchrysogenum strains (non-industrial and industrial strains with different levels of penicillin production) using 2DE-MS/MS. Our purpose was to develop a better insight into the biochemical pathways involved in penicillin production.
(2004-2006):In my M.Sc. research we performedthe molecular analysis of the clavulanic acid regulatory gene (claR gene) isolated from an Iranian strain Streptomyces clavuligerus PTCC 1709. Furthermore we studied the relationship between the length of CA repeats in the EGFR gene and the risk of breast cancer incidence and prognosis. At the same time we succeeded to develop a novel STR based molecular diagnosis of Charcot-Marie-Tooth neuropathies.

1. Production of pharmaceutical products such as Tacrolimus and Penicillin
2. Molecular cloning and site directed DNA mutagenesis
3. Genetic manipulation (gene silencing; over-expression; disruption and deletion)
4. Blotting (Southern, Western, Northern)
5. Protein biochemistry, Co-Immunoprecipitation and Immunofluorescence
6. Transformation, transfection and Oocyte injection
7. Electrophysiology
8. Cell and tissue culturing (bacterial, fungi, mammalian and plants)
9. Protein purification techniques, FPLC, HPLC
10. Electro-mobility shift assay (EMSA) and footprinting
11. Protein separation, 2-DE electrophoresis, Peptide sample preparation for Mass Spectrometry

Ph.D.- Biochemistry, M.Sc. - Plant Science from University of Lucknow. Working as Assistant Professor in Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow campus, from 2007.

Plant molecular biology, genetic engineering and protein expression and purification

My career goal is to become a principal investigator of a lab focused on finding the molecular mechanisms that regulate early adipose lineage commitment in the skin. Adipose tissue plays crucial roles in maintaining skin homeostasis. Adipose tissue in the skin produces the antibacterial peptide cathepsin in response to infection and this reaction is key to support infection clearance. During wound healing, the process of adipocyte differentiation in the skin helps recruiting fibroblasts to the wounded area; a process that promotes optimal wound healing. Both, mature adipocytes and their precursor cells have the ability to regulate hair follicle stem cell activation and thus hair growth. These findings highlight a change in the perception of the importance of adipose tissue in the skin and emphasize how little we know about the role and regulation of adipose tissue in the skin. Knowing how to influence the generation of adipose tissue in the skin will lead to the design of therapies that will range from wound treatment and infection to control of hair restoration. However, to achieve these goals we need to understand the mechanisms that regulate the balance between renewal of adipocyte precursor cells (APCs) and differentiation into mature adipocytes in the skin.
I have a strong background in molecular biology and gene transcriptional regulation that I acquired during my PhD studies in Norman Maitland's laboratory at the University of York, UK. My work there focused on finding the transcriptional mechanisms that regulated the expression of a very prostate specific gene, TGM4. I discovered that TGM4 regulation was unique from other prostate specific genes in the sense that it wasn't strongly regulated by androgenic hormones, but instead responded very strongly to retinoid acid. Using chromatin immunoprecipitation, cloning and site directed mutagenesis, I was able to identify an enhancer region containing functional retinoid acid receptor binding sites. Additionally I described an interaction between the retinoic acid receptor and the androgen receptor in regulating a set of prostate genes, implicating for the first time retinoic acid in prostate cell differentiation.
This findings led me to wonder how is differentiation regulated when transitioning from a progenitor stage to a fully differentiated cell. This interest led me to join Dr. Horsley's lab at Yale University as a postdoctoral associate. Dr. Horsley had just identified APCs in the skin and this was the perfect opportunity to satisfy my scientific curiosity using a novel model. During my postdoctoral studies I have discovered a new role for Pdgfa signaling in maintaining APCs in the skin. these experiments will shortly lead to a publication in a high-impact factor journal and have provided me with preliminary data for the research proposal. Dr. Horsley's lab is the ideal environment to conduct the first stage of the proposed experiments. We have a collaboration network that includes experts in adipose biology, chromatin structure, gene regulation and genomic analysis. I will use these support network to begin discovering the key factors involved in the commitment to the adipose lineage. I will use my preliminary data as a basis to assay whether Pdgfa signaling can modulate APC early commitment. Additionally I will use an unbiased approach that will combine known epigenetic markers and chromatin accessibility assays to delimit DNA regulatory sequences that are active at different stages of adipose lineage commitment. This will allow me to find key regulatory regions with common transcription factors. I will then test these transcription factors for their role in regulating adipose lineage commitment in APCs. These experiments could be used in the future to design therapies aimed at regulating the presence of adipose tissue in the skin with therapeutic benefits.

- Characterizing the role of Pdgfa in adipocyte stem cells in the skin.
- Genetic and epigenetic regulation of adipocyte stem cells in the skin.

Dr. Circe Mesa is the head of the Immunobiology Direction at the Center of Molecular Immunology (CIM), Cuba and leader and principal investigator of several institutional scientific projects. She is one of the responsible for the discovery and development of novel approaches and therapeutic agents for the treatment of cancer focusing on the regulation of the immune system.
Dr. Mesa joined CIM in 1997, after receiving with honors her BSc. in Biochemistry at the University of Havana. She earned her PhD in Biological Sciences in 2005 and complemented her training and formation in cancer immunology at the Edward Jenner Institute for Vaccine Research, UK and the University of Padua in Italy. Dr. Mesa has been honored with six Annual Award of the Cuban Academy of Science. She has authored or co-authored more than 30 peer-reviewed manuscripts and four patents. Dr. Mesa is member of the permanent Cuban court for doctorate degrees in Biological Sciences.

• Cancer Immunotherapy
• Tumor immunology emphasizing on its interaction with the immune system
• Myeloid derived suppressor cells (MDSC) and its interaction with tumors and the immune system.
• Development of vaccine adjuvants for the treatment of immunocompromissed patients

•Obtaining a Bachelor of Veterinary Medical Sciences, a Master and a Doctor of Philosophy degrees in Veterinary Medical Sciences (Bacteriology), Faculty of Veterinary Medicine, Zagazig University
•A Lecturer of Microbiology, department of microbiology, Faculty of Veterinary Medicine, Zagazig University, Egypt
•Teaching experience of theoretical and practical courses in Bacteriology, Mycology, Immunology and Molecular Biology
•Supervised on MVSc and PhD theses (Bacteriology, Mycology and Immunology)
•Participated in scientific workshops and seminars
•Experience in Microbiology and molecular biology techniques
•Experienced in Web-based High-throughput primer design, sequence analysi and bioinformatics
•Submission of DNA and protein sequences` data for various important genes on the GenBank
•Edition of a significant book in molecular biology
•A General Coordinator for Quality Assurance and Accreditation
•A member of Community Participation and Development Environment (Standard no 7)
•A member of the Editorial Board of several international journals
•An Associate Editor in various international journals
•A peer reviewer in many international journals
•A potential paid peer reviewer at SciRev
•Publishing many articles in impacted international journals

•Microbiology
•Diagnostic Bacteriology
•Food Microbiology
•Clinical and applied Immunology
•Mycology
•Alternative Medicine, Plant Extracts
• Gene expression
•Molecular biology
•Bioinformatics
•DNA Sequencing
•Genetics

Norhan K Abd El-Aziz is a Lecturer of Microbiology, a General Coordinator for Quality Assurance and Accreditation and a Member of Standard Society and Environment Service, Faculty of Veterinary Medicine, Zagazig University, Egypt.I obtained my MVSc and PhD degreesin Microbiology at Faculty of Veterinary Medicine, Zagazig University.I teach theoretical and practical courses in general and special bacteriology, advanced mycology, basic and clinical immunology and molecular biology. I am a supervisor on 30 post graduate students enrolled for PhD and MVSc in bacteriology, mycology and immunology topics. I attended several symposia, training courses and workshops inmolecular biology obtaining good experience in web-based high-throughput Primer Design, practical PCR, Contig Assembly DNA Sequencing,Sequence Analysis and Bioinformatics. I served as a reviewer and an Editorial Board member for several peer reviewing journals. I published many scientific papers in Microbiology, Molecular Biology, Gene expression andAlternative Medicine in many scientific journals.

Clinical Microbiology
Food Microbiology
Anaerobes
Mycoplasmas
Microbial Drug Resistance
Qualitative and Quantitative PCRs
Gene Expression
DNA Sequencing and Sequence Analysis
Herbal Extracts and Alternative Medicine
Advanced Mycology
Immunology

Yasmine H Tartor is a Lecturer of Microbiology, a General Coordinator for Quality Assurance and Accreditation and a Member of Standard Society and Environment Service, Faculty of Veterinary Medicine, Zagazig University, Egypt. I obtained my MVSc and PhD degrees in Microbiology at Faculty of Veterinary Medicine, Zagazig University. I teach theoretical and practical courses in basic and advanced mycology general and special bacteriology and clinical immunology and molecular biology. I am a supervisor on 18 post graduate students enrolled for PhD and MVSc in bacteriology, mycology and immunology topics. I attended several symposia, training courses and workshops inmolecular biology obtaining good experience in web-based high-throughput Primer Design, practical PCR, Contig Assembly DNA Sequencing, Sequence Analysis and Bioinformatics. I served as a reviewer and an Editorial Board member for several peer reviewing journals. I published many scientific papers in Microbiology, Molecular Biology, Gene expression and Alternative Medicine in many scientific journals.

Mycology (Yeasts, Dermatophytes and other molds)
Clinical Microbiology
Food Microbiology
Microbial Drug Resistance
Qualitative and Quantitative PCRs
Gene Expression
DNA Sequencing and Sequence Analysis
Herbal Extracts and Alternative Medicine
Immunology